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Methods for measuring the activity of granulocyte-macrophage colony-stimulating factor

机译:测量粒细胞巨噬细胞集落刺激因子活性的方法

摘要

In measuring the activity of the human granulocyte-macrophage colony stimulating factor, the present invention is characterized in that the monocyte hematopoietic cells isolated from the white blood cells of the chronic white blood type patient are pre-cultured in a culture medium containing human placenta culture medium, The progenitor cells were treated with human granulocyte-macrophage colony stimulating factor, and then radioactively labeled thymidine was added thereto. Then, thymidine was combined into DNA, and then the amount of radiolabeled thymidine And a method for measuring the activity of human granulocyte-macrophage colony stimulating factor.
机译:在测量人粒细胞-巨噬细胞集落刺激因子的活性时,本发明的特征在于将从慢性白血球型患者的白细胞中分离的单核细胞造血细胞在含有人胎盘培养物的培养基中预培养。用人粒细胞-巨噬细胞集落刺激因子处理祖细胞,然后向其中加入放射性标记的胸苷。然后,将胸腺嘧啶脱氧核糖核酸结合到DNA中,然后放射标记的胸腺嘧啶核苷的量和测量人粒细胞-巨噬细胞集落刺激因子活性的方法。

著录项

  • 公开/公告号KR940014810A

    专利类型

  • 公开/公告日1994-07-19

    原文格式PDF

  • 申请/专利权人 최근선;

    申请/专利号KR19920024243

  • 发明设计人 소홍섭;김인수;

    申请日1992-12-14

  • 分类号C12Q1/06;

  • 国家 KR

  • 入库时间 2022-08-22 04:37:39

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