A novel biosynthetic method for producing 7-aminodecacethoxy cephalosporanic acid

摘要

An important intermediate for the production of the cephalosporin antibiotic, 7-aminodecacethoxy cephalosporanic acid (7-ADCA), is a strain of Penicillium chrysogenecm strain transformed with adipate feedstock To produce adipoyl-6-APA (6-aminopenicillanic acid); For example, the same intracellular expression of the expandase gene from Streptomyces clavuligerus (when Penicillium chrysozoin was transformed with it) was detected in adipoyl-6-APA Is converted to adipoyl-7-ADCA by ring swelling. The final product, 7-ADCA, is then prepared by digestion of the adipoyl side chain using adipoyl acylase. Thus, the overall synthesis is carried out using biosynthetic methods and is efficient and economical.