method for the determination of an antibody in human body fluids.

摘要

An immunoassay to determine an antibody in human body fluids entails a sample containing the antibody being incubated with at least two different receptors R1 and R2, of which one receptor R1 carries a specific antigenic determinant for the antibody to be determined, and one receptor R2 carries a label, the portion which contains the bound label being separated from the portion which contains the unbound label, and the label being measured in one of the two portions. Used as control is, in place of the sample, a standard solution which contains a conjugate of a non-human antibody which is able to bind to the receptor RS specific for the antibody to be determined, or the Fab or F(ab')2 fragment thereof, and of a human immunoglobulin or its Fc portion.