Mistletoe lectin extn. - using a cationic ion exchanger for the isolation of MTLI-1 and MTL-1-2, MTLII and MTL-III, useful in bio-technology and diagnostics

摘要

The extn. of mistletoe lectins (MTL) from plant part material followed by chromatography comprises (i) pre-purificn. of an aq. extract of MTL I-III using batch absorption to a cationic exchanger, pref. SP-sephadex, (ii) biospecific absorption using lactosyl sepharose resulting in 2 fractions and (iii) sepg. the 2 fractions. Pref. the method is performed at 0-25 (pref. 4) deg.C and the extn. is performed in the presence of inhibitors, e.g. sodium sulphite and thio urea. USE/ADVANTAGE - The lectins are useful either as such or modified with other cpds. in biotechnology, analytics, diagnostics and therapy, e.g. cancer therapy as conjugates with other cpds. Sepn. of MTL-1 and MTL-2 is possible. High yields of active and stable MTL can be obtd. cheaply. Yields of up to 90% of MTL-I and 60-80% of MTL-II and MTL-III are obtd.