apparatus and method for automatic separation of extrachromosomaler dna from a cell suspension.

摘要

The invention relates to an apparatus and method for automatic purification of extra-chromosomal DNA from a cell suspension using a reaction device (1) and comprising: means (36, 21, 4, 5, 6, 34, 39) for introducing the cell suspension from a container (20) into a reaction and/or mixing chamber (33), means (2) for agitating the contents of the chamber (33), means (36, 14, 15, 4, 6, 39) for introducing a lysing solution into the chamber (33) to lyse the cells therein, means (36, 17, 18, 4, 6, 39) for introducing a precipitating solution into the chamber (33) to precipitate chromosomal DNA and, optionally, proteins and cell debris therein, means (36, 7, 8, 43) for feeding the liquid contents of the chamber (33) through a filter (31) to a collecting device (22), means (26) for purifying extra-chromosomal DNA from the contents of the collecting device (22), means (36, 25, 23, 24, 4, 5, 7, 8, 43) for introducing a dissolving solution into the chamber (33) to dissolve the precipitate remaining therein, and means (5, 6, 39, 34, 4, 16) for feeding the dissolved precipitate to waste.