Brief description of the formation of an image of the two-dimensional distribution of the ion concentration in a living cell.

摘要

The invention relates to a process of formation of the image of the two-dimensional distribution of the concentration of ions particular in a living cell, on the basis of the changes occurring in the spectrum of excitation fluorescence or of a sensor of the fluorescent. According to the process, it gives a sensor integrated in the cell, it produces an image of the distribution of the intensity of fluorescence (3) by determining the fluorescence of the sensor in a region including the cell is measured (2) is optically the cell, so that it is possible to obtain an image which can be a clear background, an image in the contrast of phase or in the interference contrast differential normaski, or an image of polarization, one determines (4) the value of the fluorescence of the bottom from a profile of the image of the fluorescence and of a profile of the image of the cell, the shape (6), an image of the two-dimensional distribution of the concentration of ions by applying an image processing of which contains the subtraction (5) of the fluorescence at the bottom of the screw - to - screw of the image of the distribution of the intensity of fluorescence, and, after processing, it displays the two images superposed (7).