Isolation, purification and utilization of sialidase-L in the synthesis of 2,7-anhydro-N-acetylneuraminic acid and for the selective cleavage of sialylamp;agr;2-3 D-galactose linkage

摘要

The instant invention relates to the isolation, purification and utilization of sialidase-L, a novel enzyme capable of producing 2,7- anhydro N-acetylneuraminic acid by the selective cleavage of sialic acid-. alpha.2→3D-galactose glycosidic bonds. The instant invention also relates to a method for the preparation of 2,7-anhydro-N-acetylneuraminic acid using sialidase-L. The instant invention also relates to methods for selectively cleaving sialic acid-&agr;2→3D-galactose glycosidic linkages and providing a means to selectively destroy the selectin ligands for the treatment of selectin-mediated inflammation. The instant invention also relates to methods for using 2,7-anhydro-N- acetylneuraminic acid and sialidase-L for the selective sialylation of acceptor glycosides to form sialic acid-&agr;2→3D-galactose glycosidic linkages.