CLONING AND EXPRESSION OF A LIPASE MODULATOR GENE FROM PSEUDOMONAS PSEUDOALCALIGENES

摘要

The present invention discloses the cloning and expression of a lipase modulator gene obtained from a class I Pseudomonas species. The expression product of the modulator gene is found to give rise to a considerable increase in lipase production especially upon homologous expression. The present invention provides a method for isolating a class I lipase modulator gene, an isolated modulator gene and a class I Pseudomonas transformed with such a gene. Finally the present invention discloses a derivative of plasmid pJRD215 which is segregationally stable in Pseudomonas.