Cell growth reactor with compartment formed by hydrophilic diaphragm and hydrophobic diaphragm

摘要

A continuous, integrated membrane bioreactor system for the culture of hybridoma or mammalian cells which meets the nutritional and physical needs of hybridomas or mammalian cells, allows for the harvesting of products, and maintains sterile integrity of the system by combining several individual apparatus having separate functions into an integrated, functioning unit. Central to this bioreactor is the compact size of the cell enclosure which is made possible by the high cell densities achieved in the layered membrane design and the continuous feed and bleed nutrient medium system which minimizes the nutrient medium volume inside the bioreactor. Maximized product output per unit of volume minimizes the space necessary to house the bioreactor. Oxygen is passed through the bioreactor as air or another gas mixture, rather than dissolved in the nutrient medium.