FOSFOMYCIN BIOSYNTHESIS-RELATED GENE AND NEW METHOD FOR PRODUCING FOSFOMYCIN WITH THE SAME

摘要

PURPOSE: To provide a fosfonycin biosynthesis-related gene for obtaining fosfomycin from 2-hydroxypropyl phosphoric acid, the fosfomycin being an antibiotic useful in the field of medical treatments. ;CONSTITUTION: The DNA contains the gene of an epoxidized enzyme which is an enzyme in the fourth stage of the biosynthesis of fosfomycin existing in an approximately 23kb gene cluster cloned from the DNA of a fosfomycin- producing bacterium and codes for an amino acid sequence of the formula. The gene can be obtained by a sub-cloning means using as a DNA donor the Streptomycin wedmorensis NP71 pFBG 23 strain (FERM P-13838) which is a strain transformed with plasmid pFBG 23 into that the gene cluster is inserted.;COPYRIGHT: (C)1995,JPO