Separated collection method null of Eli pickpocket tall from Eli pickpocket tall content

摘要

PURPOSE:To facilitate recovery of erythritol separating ability by treating a strong acidic cation exchange resin having deteriorated separating performance in treating a supernatant liquid after removing microbial cells from an erythritol-containing culture solution with a warm alkaline solution at 35-100 deg.C. CONSTITUTION:Erythritol-producing microorganisms are cultured in an aqueous culture medium under aerobic conditions to provide a culture solution. Microbial cells are then removed therefrom to afford a supernatant liquid, which is subsequently passed through a separation column filled with an alkaline metal or ammonium type strong acidic cation exchange resin. Adsorbed substances are then eluted and discharged with water to separate and collect a fraction consisting essentially of erythritol from the resultant effluent. The passage of the above-mentioned supernatant liquid and elution with water are repeated to treat the strong acidic cation exchange resin having deteriorated separating performance with a warm alkaline solution at 35-100 deg.C and recover the separating performance. The resultant resin is them reused for separating the afore- mentioned erythritol-containing fraction.