17OXYGEN LABELED PHOSPHATE COMPOUND AND METHOD FOR NUCLEAR MAGNETIC RESONANCE MEASUREMENT

摘要

PURPOSE: To obtain a ¹⁷oxygen labeled phosphate compound useful for metabolic measurement, etc., by labeling an oxygen atom of phosphate group with ¹⁷ oxygen, distinguishing a ³¹phosphorus nuclear magnetic resonance signal of a labeled phosphate group from a signal of a non-labeled phosphate group by taking advantage of polarization movement from ³¹oxygen to ³¹phosphorus to enable a selective measurement. ;CONSTITUTION: This ¹⁷oxygen labeled phosphate compound is obtained by labeling one or more oxygen atoms of a phosphate group with ¹⁷oxygen. The ¹⁷ oxygen labeled phosphate compound is introduced into a glycerophospholipid capable of forming a lipid double membrane to give a ¹⁷oxygen labeled glycerophospholipid of the formula [R' and R" each is lauric acid or myristic acid (residue)]. A phosphate group contained in a specimen is labeled with ¹⁷oxygen and polarization movement based on a spin-spin bond of ³¹phosphorus and ¹⁷oxygen are utilized so that a nuclear magnetic resonance signal of a labeled phosphate group is separated from a nuclear magnetic resonance signal of a non-labeled phosphate group and detected to preferably carry out nuclear magnetic resonance measurement.;COPYRIGHT: (C)1995,JPO