A RECOMBINING YEAST STRAIN THAT PRODUCES EXTRA-CELLULAR BOVINE RENNET AND THE PROCEDURE FOR OBTAINING IT.

摘要

Regarding Biotechnology,Especially to the field of Genetic Engineering and is aimed at obtaining a New Yeast Strain superproducer of chymosinVia recombinant.The objective is to achieve from a genetically engineered Yeast,Obtaining the Enzyme in the Culture medium and in high proportions by via fermentation.With the use of Conventional techniques of Genetic Engineering.The Gene of bovine chymosin was introduced under the promoter of Alcohol Oxidase 1 in Pichia pastoris and the signal of sucrose invertase SecretionAnd followed by the sequence of the transcription Terminator of the glyceraldehyde 3-phosphate dehydrogenase, both of sacharoimyces cerevisiaeIn a cloning vector Integration resulting from his 3 - a Mutant Yeast Strain Pichia pastoris a New metilotrofaCharacterized by the production of this proteolytic Enzyme, the digestive tract of calves.The Convention is applicable in the production of an enzyme used in the Dairy Industry for the manufacture of Cheese.!