A method for regenerating fish optic nerves originating from the preparation of water-soluble factor

摘要

A water soluble factor is derived from regenerating nerves, such as the optical nerves of fish, and this factor activates suitable target cells, such as for example glial cells, to produce extracellular proteins. Mainly laminin is thus produced. The factor is a negatively charged protein which has an apparent molecular weight of about 10,000 daltons, determined on a sieving type column. There is also provided a process for the purification of such factor. Furthermore there are provided pharmaceutical compositions which induce regeneration of nerves of the central nervous system of mammals, which contain as actaive ingredient an effective quantity of such purified facator. The effect of this factor on the regeneration of nerves is augmented when the injured site is irradiated with radiation from a low energy He-Ne laser.