Process for the preparation of a radioactive iodine-labeled TSH tracer and its use in TSH receptor assays

摘要

TSH preparation which is obtained by purification of crude bovine TSH (thyrotropin), can be converted by direct radioiodination into a radioiodinated TSH tracer for a TSH receptor assay, has a biological activity of more than 40 IU TSH/mg protein and is obtainable by purification of commercial crude bovine TSH by affinity chromatography over an anti-TSH antibody column and subsequent ion exchange chromatography over a weakly acidic cation exchanger having terminal carboxyl groups and based on a polyamide-coated silica gel, a corresponding process for its production and its use for the production of a TSH tracer, in particular for a TSH receptor autoantibody assay.