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Reagent and its use in flow cytometry for automatically determining at least one leukocyte subpopulation obtained from whole blood.
Reagent and its use in flow cytometry for automatically determining at least one leukocyte subpopulation obtained from whole blood.
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机译:试剂及其在流式细胞仪中的用途,用于自动确定从全血中获得的至少一种白细胞亚群。
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摘要
The present invention relates to a reagent and to a method of using the same for automatically determining at least one leukocyte sub-population from a total blood sample. Using this reagent, it is possible, in a single step, to lyse the erythrocytes through the action of saponin and SDS, as well as to ensure the differential staining of the leukocyte sub-populations, at the same time preserving the integrity and morphology of the cells, through the combined action of various components including chlorazol black, an alcohol, a surfactant and preserving agents. The reagent according to the invention can be applied to different types of automatic flow cytometry analyzers.
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