Procedure cloning of DNA sequences or elaboration genebank, cloning vectors and strains of microorganisms that contain them.

摘要

OBJECT OF THIS patent is a process of cloning DNA sequences or PRODUCING GENE BANKS, in which: A) SEPARATE FROM THE 5-sticky ends A TABLE CLONANTE (AS ISOLATED AFTER LINEAR DNA endonuclease treated with RESTRICCION-) phosphate groups phosphatase, ADDING TO THE iNCAPABLE OF UNION BETWEEN THEM FRAGMENTS FRAGMENTS DNA phosphate groups at their ends, cONTAINING THE gENE TO cLONING AND TO BE ACHIEVED BY TREATMENT WITH rESTRICTION-endonuclease, ligating MIXTURE ligase , OB) is transected WITH terminase a cloning vector with a cold RECOMBINANT SEQUENCE TYPE phage, cosmid, FASMID O TRANSFAMID, ASSEMBLE IN VITRO DNA monomers OBTAINED BY MEANS OF THE TWO ABOVE ALTERNATIVES. Then, they multiply the phages. They are also subject of this patent production processes CLONING VECTORS, AND CLONING VECTORS AND ORGANISMS CONTAINING.