METHOD FOR ASSAYING FOR SUBSTANCES WHICH AFFECT BCR-ABL MEDIATED TRANSFORMATION

摘要

A method for assaying for a substance that affects BCR-ABL mediated transformation which comprises reacting the substance with a first compound containing Grb2, or a portion thereof, and a second compound containing a Grb2 binding site on BCR-ABL, Sos or Shc under conditions where the first and second compounds are capable of forming a complex, and comparing with a control. The invention also relates to a method of using the substance to regulate BCR-ABL mediated transformation; and a pharmaceutical composition comprising a compound containing Grb2 or a portion thereof, the Grb2 binding site on BCR-ABL, Sos or Shc, or mimetics thereof, and a pharmaceutically acceptable carrier. Methods of treating patients suffering from CML or ALL are also contemplated.