OPERATING PROCEDURE FOR THE PRODUCTION OF VIRUSES AND VIRUS RESISTANT CELLS FOR THE TESTING OF ANTI-VIRAL SUBSTANCES USING LABORATORY ANIMALS

摘要

Viruses are produced, using laboratory animals that are transplanted with donor cells that can be infected with virus or that are infected by virus. The animals have an immune system that is distorted in such a manner that the transplanted cells are not rejected. The dose of the transplanted donor cells is sufficiently high to ensure an in vivo reaction against the recipient, preferably stimulated by the administration of donor cell growth promoting substances to the laboratory animal. The in vivo reaction against the recipient is preferably an inflammation reaction and more specifically a rejection reaction. In this manner a substantial multiplication of the donor cells is obtained. The combination of multiplication of the donor cells and the multiplication of the virus in these cells results in a very substantial virus-production. The virus production and/or the produced virus are determined and/or quantified by an in vitro virus assay, preferably in vitro tissue culture, ELISA, PCR or immunoblotting. In addition a very sensitive testing system is obtained, which can be used for the testing of anti-viral agents. Finally donor cells can be selected on virus resistance.