The present invention relates to a novel safe attenuated Pseudomonas aeruginosa strain obtained by attenuating the Pseudomonas sp. By purely isolating and purifying repeatedly according to the Fischer-Devil immunity pattern, in particular CFCPA 10142 (KCCM 10029), CFCPA 20215 (KCCM 10030), CFCPA (KCCM 10031), CFCPA 40057 (KCCM 10032), CFCPA 50243 (KCCM 10033), CFCPA 60534 (KCCM 10034) and CFCPA 70018 (KCCM 10035). The present invention also relates to a vaccine composition for preventing P. aeruginosa infection, which comprises purifying a cell wall protein having a molecular weight ranging from 10,000 to 100,000, and purifying the same.;The novel vaccine against Pseudomonas aeruginosa according to the present invention is safe without toxicity, and its cell wall protein component also exhibits a safe and excellent antibody-producing ability. Also, , It is useful for the production of a vaccine against P. aeruginosa infection.
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