The fermentation conditions for biopolymers from Zoogloea ramigera ATCC 25935 (I) consists of (1) for optimun culturing (I) in a medium of glucose 25g, dipotassium phosphate 2g, potassium phosphate 1g, sodium nitrate 0.5-1.0g, magnesium sulfate 0.2g, ferric sulfate 0.1-4.0g, yeast extract 0.01g/liter, (2) for batch controlling C/N ratio as 30-90 (glucose 90, lactose 30, molasses 60, cheese whey 60), (3) for continuous feeding medium of molasses 45.5g, sodium nitrate 1.91g, dipotassium phosphate 2g, potassium phosphate 0.5g, magnesium 0.1g/liter with dilution rate 0.048-0.402/hr. This polymer is used as aggregating agents.
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