New antifungal antibiotics, novel microorganisms of the genus Bacillus sp. Producing them and methods for producing the novel antifungal antibiotics using the same

摘要

The present invention relates to a novel Bacillus subtilis microorganism SY-414 which produces an antifungal antibiotic substance, and to an antifungal antibiotic substance produced using said microorganism Bacillus subtilis, and a production method thereof. A new Bacillus sp. Microorganism having high antimicrobial activity against plant fungi, rice blast fungi, and paperback germs is isolated from soil, cultured in YS liquid medium, centrifuged and the culture supernatant is extracted with ethyl acetate. The solvent layer is collected, After removal, the concentrated active fractions from which the solvent has been removed are collected and dissolved in methanol. After silica gel column chromatography was performed, the solvent was removed and the concentrated active fraction was dissolved in a dichloromethane: methanol (1: 5) solution to perform Sephadex LH-20 column chromatography. After removing the solvent, It is dissolved in water and lyophilized. The present invention relates to a novel antibiotic substance represented by the following general formula (I) and a production method thereof, characterized in that the antibiotic substance in powder form obtained is dissolved in methanol and then cooled to be crystallized.;Wherein R_l, R₂And R₃Are the same or different and are-H,-OH, C_One~ C₆Alkyl group, C₅~ C₂₀Of the allyl group or C₂~ C₆M, and n are each an integer of 1 to 3;