Prepn. of an anti-haemophilic factor from a cryo-precipitate

摘要

The prepn. of a concentrate of AHF (antihaemophilic factor-Factor VIII) from a cryoppte. comprises: (a) suspending and dissolving the cryoppte.; (b) removing non-AHF proteins and precipitating AHF in a known manner to give a crude AHF paste as a sediment; (c) carrying out a first virus inactivation of the crude AHF paste by heat treatment in the presence of 0.7-1.4 g/ml calcium gluconate and 1.25-1.5 g/ml sucrose pooled concentrate and - 0.5 mol/l of other protein-stabilising substances chosen from the amino acids glycine, alpha or beta alanine, lysine, leucine, valine, asparagine, serine, hydroxyproline, proline, glutamine, alpha or beta or eta -aminobutyric acid; (d) cooling and diluting with distilled water; (e) carrying out a second virus inactivation using a solvent-detergent (SD) process; (f) purifying the extract by chromatography using a weakly basic anion exchange resin; and (g) ultra filtering and diaconcentrating the extract.