A method for the production of recombinant human gamma - interferon without without the methionine, cysteine, and at the n - terminal.

摘要

The method is used for the preparation of the -interferon medicament. It ensure product yield exceeding 1.109 U/l culture, and furthermore no momoclonal antibodies are used for its treatment. The -interferon, the N-end methionone of which is intercellularly removed has purity over 99.6%, preserves its biological activity after freeze-drying and is soluble in water solutions. The method is effected when plasmide pJMB-carrier of synthetic gene of recombinant human non-cystein -interferon is expressed in strain Escherichia coli LE 392, registered in NBIMCC under No. 845, and is cultivated in a fermenter at 36-38°C under conditions of aeration. The turned out product is purified, renaturated and eluated, and the accompanying solvents and impurities are removed.