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Retroviral vector for the transfer and expression of genes in eukaryotic cells.
Retroviral vector for the transfer and expression of genes in eukaryotic cells.
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机译:逆转录病毒载体,用于在真核细胞中转移和表达基因。
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摘要
The invention relates to a recombinant vector for the cloning and / or the expression and / or the transfer of an exogenous nucleotide sequence, characterized in that it comprises any sequence contained in the ClaI-PvuII fragment comprising the nucleotides 7702 and 1527 of the sequence given in Figure 1 and comprising the LTR sequence between nucleotides 7842 and 144, the PBS site starting at nucleotide 145, the encapsidation sequence included in the sequence of 250 nucleotides following the end of the LTR sequence, said sequence being capable of controlling cloning and / or expression and / or transfer of the exogenous sequence, irrespective of its transcriptional orientation with respect to the orientation or transcription of the virus. BR/ It relates to the use of this vector for the transfer and / or cloning and / or expression of genes, in particular in the context of gene therapy. / P
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