Size separation of particles contained within a material by the use of nonaqueous hydrodynamic chromatography

摘要

A method is disclosed for determining the distribution according to size of particles which make up a noncontinuous phase dispersed within a matrix which is not completely soluble in an aqueous solution. The method involves dissolving the matrix containing the particles in an essentially nonaqueous liquid causing the particles to be suspended. The suspension is then eluted through a separating medium which sorts the particles according to size. A detector monitors the effluent from the separating medium and creates a record of the data. This record is then compared to data obtained from known standards in order to estimate the size distribution in the sample.