Method and devices for delivery of substrate for the detection of enzyme- linked, membrane-based binding assays

摘要

The invention provides a method for supplying an enzyme substrate to a membrane-based, enzyme-linked reaction, comprising providing an open pore, high liquid retention capacity material impregnated with a predetermined amount of a substrate for the enzyme; and contacting the material with a membrane containing the enzyme-linked reaction under conditions which permit diffusion of the enzyme substrate to sites on the membrane containing the enzyme linked reaction. The invention further provides devices for membrane-based enzyme-linked reactions comprising a porous material impregnated with a predetermined amount of a substrate for the enzyme, wherein the porous material is paper, glass fiber paper, stainless steel mesh, woven and nonwoven nylon, polyester, polyethylene, polypropylene, or fluorocarbon polymers having a porosity ranging from about 5 microns to 5000 microns; and a microporous membrane of cellulose or a cellulose derivative, nylon, polysulfone, polypropylene, PTFE, or PVDF having a porosity ranging from about 0.05 microns to about 12 microns.