Its manufacture and substituted indoles using the kit and method for performing colorimetric method, the method of 4-hydroxy-2-ene aldehyde and malondialdehyde as an indicator of lipid peroxidation

摘要

(57) The method of the present invention summary of the formula in the medium to be (a) Analysis: (In the formula, C and A are the same or different, H it it, Shows, but never show simultaneously H, B is Adding a compound represented by) is; acidifying the medium hydrochloride (b) or perchloric acid, or sulfonic acid or carboxylic acid strength, sulfate or hydrobromic acid; the medium was (c) acidic reduction , and incubated at 25~60 ℃ in order to obtain a stable color development; measuring the absorbance of the medium that the chromogenic at absorption wavelength specific to the chromophore adduct was (d) generation, was used, and the measurement and is characterized in that they are used to determine MDA concentration and 4-hydroxy-2-ene in accordance with the aldehyde acid. Assays indicator of lipid peroxidation, and its application to clinical research in particular.