CELL CLONE HAVING HUMAN ABORTION-ASSOCIATED FETAL ANTIGEN DERIVED FROM B-LYMPHOCYTE STRAIN ORIGINATING FROM PATIENT WITH BLOOM'S SYNDROME AND DIAGNOSIS BY MEASURING ABORTION-RELATING FETAL ANTIBODY IN SERUM OF ABORTION PATIENT AT EARLY STAGE OF PREGNANCY USING THOSE CELL MEMBRANE PROTEINS AND ABORTION SUPPRESSING AGENT

摘要

PURPOSE: To obtain a cell clone having a human abortion-associated feral antigen derived form a B-lymphocyte strain originating form a patient with Bloom's syndrome and to provide a diagnostic method for measuring the abortion-associated fetal antibody relating to the abortion at early stage of pregnancy, the judgment of the effect after immunological therapy with husband lymphocytes, and an abortion suppressing agent. ;CONSTITUTION: A cell clone having a human abortion-associated fetal antigen obtained by TPA treating by a B-lymphocyte from the blood of a Bloom syndrome patient with EB virus-sensitized high frequency sister chromatid exchanged character, cell surface IgM and Pre-B lymphocyte plasma cell a diagnostic method for examining the presence of the human abortion-associated fetal antibody in the serum of a woman experiencing an early-stage abortion through a fluorescent antibody technique by determining a product from the antigen- antibody reaction between this cell clone and her serum specimen, a diagnostic reagent therefrom for this antibody by allowing the cell clone to react with the serum positive to this antibody, selectively collecting only a large amount of the antigen-positive cells by the panning method to recover the cell membrane proteins, solubilzing the proteins and allowing this antigen to react with a serum to be examined according to the WB method, and a therapeutic agent based on this diagnostic method.;COPYRIGHT: (C)1996,JPO