INFECTIOUS cDNA-CLONE OF TICK ENCEPHALITIS (TBE), VACCINE DERIVED THEREFROM AND PROCESS FOR PREPARING SUCH VACCINE

摘要

New complete cDNA (I), encoding an infectious tick-borne encephalitis virus (TBEV) RNA transcript is: (a) a 11141 bp sequence reproduced in the specification; or (b) a sequence with at least 70 (esp. 95)% homology with (a). Also new are: (1) cDNA (Ia) encoding infectious TBEV-RNA transcripts contg. the 3'-non-coding region (i.e. bases 10375-11141) of (a) plus the TBEV-coding sequence of a suitable TBEV strain; (2) cDNA (Ib) encoding infectious TBEV-RNA transcripts comprising the TBEV-coding part of (a) plus a 3'-non-coding sequence of a related flavivirus; (3) infectious RNA transcripts of (I), (Ia) or (Ib), produced in vivo or in vitro, (4) recombinant DNA consisting of (I), (Ia0 or (Ib), plus a vector and a promoter, (5) transformed/transfected prokaryotic or eukaryotic cells contg. such recombinant DNA.