METHOD FOR ISOLATING LIPOXIGENASE-1 FROM PLANT SEEDS

摘要

the object of the invention is lipu0151xigenu00e1zu20131 nu00f6vu00e9nyimagvakbu00f3l enzyme separation procedure.according to the invention hu0151gy act, the plant produced magvakbu00f3lu0151rlemu00e9nyt, known mu00f3du0151n five - and ten times tu00e9rfu0151gatu00fa, aqueous acidic u0151ldattal 0.5-1 hours 4 u0159c - you are stirring, kivu0151natu0151lju00e1k,i'll azu00edgy won kivu0151natu0151t 4,5u20136,0 ph, preferably ph 5,1u20135,3, 0,05u20130,15iu0151neru0151ssu00e9gu0171 0,05 iu0151neru0151ssu00e9g medium, preferably 65 to 70 u0159c - you are, preferably, 70 u0159c for 3 to 10 minutes, s appropriate, is heat treated for 5 minutes. ahu0151kezelt mixture cooled centrifu0171gu00e1lju00e1k or filtered.the nyertu0151ldatu0151t or used directly or frozen tu00e1ru0151lju00e1k, or a pu0151rlasztva frozen or dried.