New temperature-modulated DNA constructs for the controlled production of recombinant proteins, modified microorganisms containing them and processes using these microorganisms

摘要

DNA construct, comprising a DNA coding for a protein which can be produced by a microorganism and at least one promoter sequence, characterised in that between the promoter and the gene of the recombinant protein which has to be expressed there is at least one possibly mutated transcription terminator of the RO-independent and temperature-sensitive type. These temperature- modulated DNA constructs make it possible to obtain almost absolute control over the rate of production of recombinant proteins. Figure 3 shows for example the kinetics of the production of chloramphenicol acetyl- transferase (CAT) in E. coli modified with constructs containing different forms of the T1 terminator of rrnB. ^ CAT enzyme produced (units/mg of total cell proteins) growth time in fresh medium (min). ^CAT enzyme produced (% of total cell proteins) IMAGE