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Procedure specifies replacement of a copy of a gene present in the host genome by integrating a different gene than that in which the integration has been performed.
Procedure specifies replacement of a copy of a gene present in the host genome by integrating a different gene than that in which the integration has been performed.
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机译:该规程规定了通过整合与已经进行整合的基因不同的基因来替换宿主基因组中存在的基因的副本。
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摘要
The invention relates to the insertion of foreign DNA OBJECT IN A CHOSEN IN THE GENOME OF A CELL SITE eucaryote. THE CHOSEN SITIDO INSERTION IS IN A GENE. OBJECT INSERTION is performed eucaryotes TRANSFERRING CELLS WITH A vector containing the foreign DNA inserted flanked by two STREAMS ARE ADJACENT TO genomic insertion site RECEIVER DESIRED IN GEN. INSERTING THE DNA OF MAY LEAD TO BE A coding sequence, BE A regulatory sequence. STRINGS Flanking ARE PICKED IN ORDER TO PERMIT FOR Homologous Recombination, where appropriate, be the expression of the coding sequence DNA INSERCION UNDER CONTROL SEQUENCES REGULATING GENE recipient, is the expression of a coding sequence GENE RECEIVER LOW SEQUENCE CONTROL OF REGULATING insert DNA.
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