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Process for the conversion of a fused protein active human motilin analogues and purifying these analogs.
Process for the conversion of a fused protein active human motilin analogues and purifying these analogs.
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机译:转化融合蛋白的活性人胃动素类似物并纯化这些类似物的方法。
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摘要
PROCESS FOR CONVERSION IN PROTEIN FUSED HUMAN motilin analogues, active type, and purify the like. DESCRIBED PROCESS TO CONVERT A PROTEIN FUSED CONTAINING motilin analogues HUMAN DOBLEMENTE REPEATED STATES TO a methionine residue BETWEEN motilin analogues NEIGHBOR, expressed in a microorganism RESPECT analogs biologically active motilin BY SEPARATION CHEMISTRY OF PROTEIN FUSED Y Purification of the motilin analogues biologically active. FUSED PROTEIN EXPRESSED IN SPREADING TREATING TRANSFORMED MICROORGANISM it with cyanogen bromide in an aqueous solution of formic acid. After neutralization solution RESULTING REACTION ARE HOLDING THE CLEANSING IN COMBINATION WITH OPERATIONS OPERATIONS distillation and hydrophobic chromatography, chromatographs ion exchange and reverse phase.
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