PCR AMPLIFICATION OF REARRANGED GENOMIC VARIABLE REGIONS OF IMMUNOGLOBULIN GENES

摘要

The invention features methods for isolating immunoglobulin variable regiongenes, and the use of these genes in the production of chimeric and isotypeswitched antibodies. The invention also features substantially pure DNAencoding a variable region of the antibody produced by hybridoma cell line HNK-20, and chimeric antibodies containing this variable region.