LOW MOLECULAR WEIGHT THERMOSTABLE β-D-GLUCOSIDASE FROM ACIDOTHERMUS CELLULOLYTICUS

摘要

A purified low molecular weight β-D-glucosidase is produced from Acidothermus cellulolyticus ATCC 43068. The enzyme is water soluble, possesses activity against pNP-β-D-glucopyranoside, has a high degree of stability toward heat, and exhibits optimal temperature activity at about 65 °C at a pH range of from about 2 to about 7, has an inactivation temperature of about 80 °C at a pH range of from about 2 to about 7 and has a molecular weight of about 50.5-54.5 as kD determined by SDS-PAGE.