ASSAY FOR MARKER OF HUMAN POLYMORPHONUCLEAR LEUKOCYTE ELASTASE ACTIVITY

摘要

A radioimmunoassay (RIA) based on the Aα(Val360) epitope which allows the evaluation of the potency of compounds that inhibit formation of cleavage products containing this neoepitope in a variety of in vitro cell biological situations is provided. The new RIA may be to detect an endogenous Aα(Val360) signal in normal human plasma and at elevated levels in cystic fibrosis plasma and in rheumatoid arthritis synovial fluid samples. The assay procedure is a single step assay which allows for the rapid and reproducible detection of specific cleavage peptides.