PRODUCTION OF BIOLOGICALLY ACTIVE INSULIN-LIKE GROWTH FACTOR I FROM HIGH EXPRESSION HOST CELL SYSTEMS

摘要

new method of lower igf-i levels decreased. the method is implemented by providing additional positive charge at the end of the n-terminus of the molecule of igf-i in the presence of a sequence of short lead line.it was found that the positive charge in the end is the new molecule of igf-i is recombinant igf-i to withdraw by simply stirring solubilized inclusion body protein for 2 to 16 hours, or during the night. the invention relates to a fusion protein nouel through igf-i, attached to the head - of - line sequence is positively charged.