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SUBSTRATE-FIXED PENICILLIN G AMIDASE, GLUTARYL-7-ACA ACYLASE OR D-AMINOACID OXIDASE
SUBSTRATE-FIXED PENICILLIN G AMIDASE, GLUTARYL-7-ACA ACYLASE OR D-AMINOACID OXIDASE
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机译:基质固定的青霉素G酰胺酶,谷氨酰7-ACA酰化酶或D-酰胺酸氧化酶
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PCT No. PCT/EP94/04132 Sec. 371 Date May 30, 1996 Sec. 102(e) Date May 30, 1996 PCT Filed Dec. 13, 1996 PCT Pub. No. WO95/16773 PCT Pub. Date Jun. 22, 1995An enzyme selected from penicillin-G amidase, glutaryl-7-ACA acylase and D-amino acid oxidase is immobilized by covalent bonding on an aminofunctional organosiloxane polymer carrier to provide an immobilized enzyme having a specific volume activity of at least 100 U/g wet carrier. Preferably, the carrier has an average diameter or 0.01 to 3 mm and is essentially spherical. Covalent bonding is accomplished by activating amino groups on the carrier with a dialdehyde and reacting the activated groups with reactive groups on the enzyme. An amount of enzyme is immobilized to provide a weight ratio of enzyme to carrier of 1 to 300 mg protein per g wet carrier.
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