Preparation of human insulin precursor by genetic recombination and preparation of insulin using the same

摘要

The present invention relates to a method for introducing a vector obtained by inserting a DNA fragment of tumor necrosis factor alpha into a vector as a fusion partner and inserting a DNA coding for an insulin precursor having the following general formula into a vector, The present invention relates to a method for producing an insulin precursor having the following general formula, characterized by separating and purifying a culture.;B chain-X-A chain In the above formula, X has the following formula. C: proline; D: glycine; arginine or lysine; E: unspecified amino acid sequence; and n is 0 to 5.