DOMAIN I OF PROTEASE INHIBITOR, DNA, VECTOR, CELL, METHOD OF PROTEASE INHIBITOR PREPARING, PHARMACEUTICAL COMPOSITION, THE USE OF DOMAIN I

摘要

FIELD: biochemistry, molecular biology. SUBSTANCE: variant of domain I of human protease inhibitor of Kunitz type has the following amino acid sequence: X¹-Cys-Ala- -Phe-Lys-Ala-Asp-X²-Gly-X³-Cys-X⁴, X⁵, X⁶, X⁷, X⁸, X⁹-Phe-Phe-Phe-Asn-Ile-Phe- -Thr-Arg-Glu-Cys-Glu-Glu-Phe-X¹⁰-Tyr-Gly-Gly-Cys-X¹¹, X¹², X¹³-Gln-Asn- -Arg-Phe-X¹⁴-Ser-Leu-Glu-Glu-Cys-X¹⁵, X¹⁶-Met-Cys-Thr-Arg-X¹⁷ (sequence D N1) where X¹ means H or 1-7 natural amino acid residues for exception Cys; every of X²-X¹⁶ independently means natural amino acid residue and X¹⁷ means OH or 1-5 natural amino acid residues for exception Cys; condition: at least one of amino acid residues X¹-X¹⁷ distincts of the corresponding amino acid residue of natural sequence. EFFECT: improved method of inhibitor preparing.