STRAIN OF FUNGUS TOLYPOLADIUM INFLATUM SUBSP BLASTOSPORUM - A PRODUCER OF CYCLOSPORINE A AND A METHOD OF CYCLOSPORINE A BIOSYNTHESIS

摘要

FIELD: microbiological industry. SUBSTANCE: strain Tolypoladium inflatum subsp. blastosporum 1069 is prepared by selection and deposited at number ВНИИА N 319 A. For method realization strains ВНИИА N 331 A or ВНИИА N 330 A are also used except for the strain 1069. Producer is grown on the sowing medium of the following composition, wt. -%: soybean flour 2-3; glycerol 2.5-3.5; molasses 2.0-2.5; potassium dihydrogen phosphate 0.3-0.6; sodium chloride 0.3-0.6, and water the rest, medium pH is 6.5-6.7. Obtained mycelium is used as inoculate for biosynthesis. For fermentation carried out under aeration and stirring conditions at 23-25 C the fermentation medium of the following composition is used, wt.-%: molasses 12-20; glycerol 0.5-1.5; organic nitrogen source 0.4-1.5 (БВК 0.4-0.8 wt.-%, soybean flour 0.5-1.5 wt. -%, corn extract 0.5-1.0 wt.-% or their mixture at the total concentration 0.8-1.5 wt. -%), potassium dihydrogen phosphate or ammonium hydrogen phosphate 0.5-0.8; cobalt chloride 0.0003-0.0006, and water the rest, medium pH is 6.0-6.5. Activity of cultural fluids are 750-800, 550-800 and 120-600 mcg/ml using strains 319A, 330A and 331A, respectively. EFFECT: increased yield of the end product. 5 cl, 3 tbl