首页>
外国专利>
Process for the purification of vitamin K-dependent coagulation factors by chromatography
Process for the purification of vitamin K-dependent coagulation factors by chromatography
展开▼
机译:色谱法纯化维生素K依赖性凝血因子的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
A process for the purification of vitamin K dependent coagulation factors by affinity chromatography wherein fractions of a source containing said vitamin K dependent factors are a) subjected to chromatography on a material modified with 2-hydroxyaminopropyl groups bonded to spacers having more than 3 carbon atoms, said source being applied from a solution whose ionic strength is chosen such that said vitamin K dependent coagulation factors are adsorbed on the above anion-exchange material, washing is optionally performed with an appropriate buffer solution, then, after separation of the eluate, b) a buffer solution of higher ionic strength than that used in step a) is contacted with the affinity chromatographic material such that said vitamin K dependent factors are eluted, then this fraction is either processed further to give a PPSB preparation, or these fractions containing the factors are pooled and then said fractions c) are subjected to a second chromatographic step in the form of a heparin affinity chromatography.
展开▼