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Process for the preparation of pyruvic acid using permeabilized transformants of H. polymorha and P. pastoris which express glycolate oxidase and catalase
Process for the preparation of pyruvic acid using permeabilized transformants of H. polymorha and P. pastoris which express glycolate oxidase and catalase
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机译:使用表达乙醇酸氧化酶和过氧化氢酶的多摩链霉菌和巴斯德毕赤酵母的透化转化体制备丙酮酸的方法
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摘要
A process for the production of pyruvic acid involving the enzymatic reaction of L-lactic acid and oxygen in an aqueous solution in the presence of catalysts glycolate oxidase ((S)-2-hydroxy-acid oxidase, EC 1. 1.3.15) and catalase (EC 1.11.1.6). High yields of pyruvate at high purity can be achieved at commercially acceptable concentration without substantial product inhibition of the enzyme.
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