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Spectrophotometric quantitation of images in X-ray film and electrophoresis gel bands
Spectrophotometric quantitation of images in X-ray film and electrophoresis gel bands
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机译:X射线胶片和电泳凝胶带中图像的分光光度定量
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摘要
Images comprised of silver such as X-rays and electrophoresis gel bands, wherein the size and darkness of the image provides useful information, are quantitated utilizing a spectrophotometer to quantify the silver which comprises the image. An image is isolated from X-ray film or an electrophoresis gel followed by removal therefrom of any silver which is present. The removed silver is complexed to cause an optical change. A spectrophotomer is then used to measure the absorbance and the absorbance measurement is compared with a calibrated reference standard.
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