Method of measuring the change metaplasia of mucus-secreting epithelial cells

摘要

(57) how to quickly assess the changes in hypertrophy and hyperplasia in [summary] animal respiratory tract, in particular, is an assay that measures the neutral mucopolysaccharides protein acidic and linearly at least 10μg or 0.5μg. The homogenized in a solution which is suitably buffered to be exposed to an inducer suspected test animals, and lungs were removed, reducing agent and containing protease inhibitors, to remove particulate matter, the assay, SDS treatment is made of the size fractionation of the soluble extract was. The immobilized high molecular weight materials, each stained neutral mucopolysaccharide substances or acidic, to quantify the specific staining. Changes observed are consistent with those observed in the histological sections of tissue exposure. And to verify the metaplastic potential of a compound suspected, in order to determine neurohumoral mediators of which one to participate in the mucus cell metaplasia in an animal model of chronic obstructive pulmonary disease for the assay, and It is useful to identify compounds that may reduce these effects.