HUMAN 26S PROTEASOME-CONSTITUTING COMPONENT PROTEIN AND DNA CODING THE SAME

摘要

PROBLEM TO BE SOLVED: To obtain a new protein having a specific amino acid sequence, constituting a human intracellular protease, that is human 26S proteasome, and useful for clarifying the function of the proteasome, for treating, diagnosing, etc., various kinds of diseases such as Alzheimer disease. SOLUTION: This new protein is a component constituting the human 26S proteasome containing the amino acid sequence of the formula. The new protein has an important role in the intracellular protein decomposition system, and is useful for the clarification of the function of the human 26S proteasome, for the cause clarification, therapy and diagnosis of Alzheimer disease, etc. The new protein is obtained by culturing human lymphoma strain U937 in a culture medium containing 5% bovine fetal serum in a 5% CO2 gas flow at 37 deg.C, collecting the cells, separating the mRNA by a conventional method, producing a cDNA library by the use of the mRNA, screening the cDNA library with a probe synthesized on the basis of the base sequence of the human 26S proteasome-constituting component protein, joining a gene obtained from the positive clone with a vector, and subsequently expressing the new protein in host cells.