SYNTHESIS OF BORON TRIFLUORIDE COORDINATED COMPOUND AND OLIGONUCLEOTIDE

摘要

PURPOSE: To obtain a new coordinated compound having an activity as a reaction accelerator in a phosphoramidite method higher than 1H-tetrazole, large solubility in an organic solvent, and combinedly having profitability and simplicity. ;CONSTITUTION: A boron trifluoride-benzimidazole complex of the formula is obtained by reacting (A) an ether complex obtained by dissolving boron trifluoride in an ether such as diethyl ether or tetrahydrofuran at room temperature with (B) benzimidazole in (C) a halogenated alkane solvent such as dichloromethane, chloroform, carbon tetrachloride or 1,2-dichloroethane in a molar ratio of A:B=1:1. The second objective oligonucleotide is obtained by reacting nucleoside phosphoramidite or nucleoside phosphonoamidite with nucleoside in the presence of the complex.;COPYRIGHT: (C)1996,JPO