HUMAN AMNIOTIC CELL AND CELL FOR GENE THERAPY CONSISTING OF THE SAME

摘要

PROBLEM TO BE SOLVED: To obtain the subject new cell capable of transducing a foreign gene effective for, e.g. therapy of genetic diseases involved in gene deletion, deficiency or abnormalities into a patient without rejection by transducing a desired gene hoped to be manifested and manifesting a product of the gene. SOLUTION: This new human amniotic cell is obtained by transducing, e.g. a recombinant adenovirus vector prepared by integrating an adenovirus vector with a desired gene hoped to be manifested, capable of manifesting a product coded with the gene. The amniotic cell is capable of transducing a foreign gene effective for e.g. therapy of genetic diseases involved in gene deletion, deficiency or abnormalities into a patient without any rejection, therefore being useful for gene therapy. The human amniotic cell is obtained by the following process: amniotic epithelium is separated from a placenta derived from cesarean section, etc., cut into several fragments, which are put to trypsin treatment to separate cells, which are collected by centrifugal separation and then cultured in a medium in the presence of 5% CO2 , and the resultant cells are tranduced with a recombinant adenovirus vector containing a gene hoped to be manifested by e.g. electroporation.