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Is used for the simple identification manner and this identification manner of the lactobacillus the identification kit
Is used for the simple identification manner and this identification manner of the lactobacillus the identification kit
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机译:用于乳酸菌的简单鉴定方式和该鉴定方式的鉴定试剂盒
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摘要
PURPOSE: To simply and accurately identify lactic acid bacteria without using a reference sample by cleaving a ribosomal RNA gene of lactic acid bacteria with a restriction enzyme, analyzing length of the resultant DNA fragment and comparing the analyzed length with the length of a standard DNA. ;CONSTITUTION: A ribosomal RNA gene of lactic acid is cloven with a specific restriction enzyme HinfI, DraI, etc. Then, the length of the produced DNA fragment is analyzed and compared with a standard DNA strain to identify strain of lactic acid bacteria. Concretely, e.g. 16Sr DNA is specifically amplified from a chromosomal DNA by an enzymatic gene amplifying method and cloven with a restriction enzyme and then subjected to agarose gel electrophoresis and a test bacterium exhibiting electrophoresis pattern which coincides with a standard strain is identified as same bacterial strain as the standard strain.;COPYRIGHT: (C)1993,JPO&Japio
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